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qscripttm microdna cdna synthesis kit  (Quanta Biosciences)


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    Quanta Biosciences qscripttm microdna cdna synthesis kit
    Qscripttm Microdna Cdna Synthesis Kit, supplied by Quanta Biosciences, used in various techniques. Bioz Stars score: 96/100, based on 288 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/qscript+flex+cdna+kit/qScript+Flex+cDNA+Synthesis+Kit/10__1016_slash_j__omtn__2026__102927-240-8-13
    Average 96 stars, based on 288 article reviews
    qscripttm microdna cdna synthesis kit - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Reverse Transcription:

    Article Title: Systematic analysis of the target recognition and repression by the Pumilio proteins
    Article Snippet: .. RNA was reverse transcribed using the qScript Flex cDNA kit (Quanta bioscience) with a mix of oligo dT and random primers according to the manufacturer's instructions. .. Gene expression was determined using the ViiA7 qPCR with the Fast SYBR qPCR mix (Life Technologies).

    Article Title: Inhibition of miR-20a promotes neural stem cell survival under oxidative stress conditions
    Article Snippet: RNA was isolated using the standard TRIzol protocol (Sigma). .. Reverse transcription (RT) reactions were conducted using the qScript Flex cDNA Kit (Quantabio) in a total reaction volume of 10 μL. ..

    Article Title: Polypeptide and related products, methods and uses
    Article Snippet: PCR and Reverse Transcription Quantitative PCR (RT-qPCR) DNA or plasmid vector were PCR amplified using Q5 High-Fidelity 2×Master Mix (Bio Labs, M0492S) and target specific primers (IDT) following manufacturer's instructions. .. RNA (50-500 ng) was reverse transcribed to cDNA using qScript Flex cDNA Kit (Quantabio, 95049-025) with a combination of random primers and oligo (dT). ..

    Article Title: Illumina MiSeq and iSeq Platforms Yield Comparable Results for Viral Genomic Sequencing.
    Article Snippet: Summary: Illumina MiSeq and iSeq are widely used short-read next-generation sequencing (NGS) platforms.. The MiSeq instrument is specialized for mid-range throughput, while the iSeq instrument has lower throughput but is less expensive and simpler to operate.. Several studies have compared Illumina platforms for sequencing of a variety of specimen types, but very few have quantified differences in sequencing quality, particularly for viruses.

    Multiplex Assay:

    Article Title: Analysing the loss of embryogenic competence in long-term cell lines of Solanum betaceum Cav.: involvement of miR827, phosphate and sugar.
    Article Snippet: .. For miR827 quantification, the two-tailed RT reactions were performed with the qScript Flex cDNA Kit (Quantabio, Massachusetts, USA) in multiplex, whenever possible. ..

    Article Title: Analysing the loss of embryogenic competence in long-term cell lines of Solanum betaceum Cav.: involvement of miR827, phosphate and sugar
    Article Snippet: .. For miR827 quantification, the two-tailed RT reactions were performed with the qScript Flex cDNA Kit (Quantabio, Massachusetts, USA) in multiplex, whenever possible. ..

    cDNA Synthesis:

    Article Title: The snakehead retrovirus promoter functions independently of the 3’ORF protein and its products are maternally inherited in transgenic zebrafish
    Article Snippet: .. First-strand cDNA synthesis was performed with qScript Flex cDNA Kit (Quantabio, 95049) and random primers. ..

    Article Title: The snakehead retrovirus promoter functions independently of the 3'ORF protein and its products are maternally inherited in transgenic zebrafish.
    Article Snippet: .. First-strand cDNA synthesis was performed with qScript Flex cDNA Kit (Quantabio, 95049) and random primers. ..



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    qscripttm microdna cdna synthesis kit  (Quanta Biosciences)
    96
    Quanta Biosciences qscripttm microdna cdna synthesis kit
    Qscripttm Microdna Cdna Synthesis Kit, supplied by Quanta Biosciences, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/qscript+flex+cdna+kit/qScript+Flex+cDNA+Synthesis+Kit/10__1016_slash_j__omtn__2026__102927-240-8-13
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    qscripttm microdna cdna synthesis kit - by Bioz Stars, 2026-09
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    qscript flex cdna synthesis kit  (Quanta Biosciences)
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    RT-qPCR to assess the expression of the reported genes after ( A ) transfection with siRNAs against EPB41L4A-AS1, ( B ) CRISPRa with guides targeting the EPB41L4A-AS1 promoter, ( C ) transfection with plasmid encoding the EPB41L4A-AS1 <t>cDNA,</t> transfection with GapmeRs ( D ) and siRNAs ( E ) targeting EPB41L4A, ( F ) CRISPRa with guides targeting the EPB41L4A promoter, and ( G ) transfection with plasmid encoding the EPB41L4A cDNA. ( H ) Changes in EPB41L4A-AS1 expression after rescuing EPB41L4A-AS1 with an ectopic plasmid or CRISPRa following its KD with GapmeRs. In both panels (Ectopic OE and CRISPRa) the ‘-’ samples represent those transfected with the Empty Vector or sgControl. Asterisks indicate significance relative to the –/– control (transfected with both the control GapmeR and vector). ( I ) Same as in ( H ), but for changes in EPB41L4A expression. ( J ) UMI-4C contact profiles in control and LNA2-transfected cells using baits targeting the TSS of EPB41L4A-AS1. The green area represents the quantified genomic interval, and the p-value was calculated using a Chi-squared test. All experiments were performed in n = 3 biological replicates, except UMI-4C with n = 2, with the error bars in the boxplots representing the standard deviation. In all cases, ns = p > 0.05; *p < 0.05; **p < 0.01; ***p < 0.001 (two-sided Student’s t -test).
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    quantabio qscript ultra  (Quanta Biosciences)
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    RT-qPCR to assess the expression of the reported genes after ( A ) transfection with siRNAs against EPB41L4A-AS1, ( B ) CRISPRa with guides targeting the EPB41L4A-AS1 promoter, ( C ) transfection with plasmid encoding the EPB41L4A-AS1 <t>cDNA,</t> transfection with GapmeRs ( D ) and siRNAs ( E ) targeting EPB41L4A, ( F ) CRISPRa with guides targeting the EPB41L4A promoter, and ( G ) transfection with plasmid encoding the EPB41L4A cDNA. ( H ) Changes in EPB41L4A-AS1 expression after rescuing EPB41L4A-AS1 with an ectopic plasmid or CRISPRa following its KD with GapmeRs. In both panels (Ectopic OE and CRISPRa) the ‘-’ samples represent those transfected with the Empty Vector or sgControl. Asterisks indicate significance relative to the –/– control (transfected with both the control GapmeR and vector). ( I ) Same as in ( H ), but for changes in EPB41L4A expression. ( J ) UMI-4C contact profiles in control and LNA2-transfected cells using baits targeting the TSS of EPB41L4A-AS1. The green area represents the quantified genomic interval, and the p-value was calculated using a Chi-squared test. All experiments were performed in n = 3 biological replicates, except UMI-4C with n = 2, with the error bars in the boxplots representing the standard deviation. In all cases, ns = p > 0.05; *p < 0.05; **p < 0.01; ***p < 0.001 (two-sided Student’s t -test).
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    Image Search Results


    RT-qPCR to assess the expression of the reported genes after ( A ) transfection with siRNAs against EPB41L4A-AS1, ( B ) CRISPRa with guides targeting the EPB41L4A-AS1 promoter, ( C ) transfection with plasmid encoding the EPB41L4A-AS1 cDNA, transfection with GapmeRs ( D ) and siRNAs ( E ) targeting EPB41L4A, ( F ) CRISPRa with guides targeting the EPB41L4A promoter, and ( G ) transfection with plasmid encoding the EPB41L4A cDNA. ( H ) Changes in EPB41L4A-AS1 expression after rescuing EPB41L4A-AS1 with an ectopic plasmid or CRISPRa following its KD with GapmeRs. In both panels (Ectopic OE and CRISPRa) the ‘-’ samples represent those transfected with the Empty Vector or sgControl. Asterisks indicate significance relative to the –/– control (transfected with both the control GapmeR and vector). ( I ) Same as in ( H ), but for changes in EPB41L4A expression. ( J ) UMI-4C contact profiles in control and LNA2-transfected cells using baits targeting the TSS of EPB41L4A-AS1. The green area represents the quantified genomic interval, and the p-value was calculated using a Chi-squared test. All experiments were performed in n = 3 biological replicates, except UMI-4C with n = 2, with the error bars in the boxplots representing the standard deviation. In all cases, ns = p > 0.05; *p < 0.05; **p < 0.01; ***p < 0.001 (two-sided Student’s t -test).

    Journal: eLife

    Article Title: EPB41L4A-AS1 long noncoding RNA acts in both cis - and trans -acting transcriptional regulation and controls nucleolar biology

    doi: 10.7554/eLife.106846

    Figure Lengend Snippet: RT-qPCR to assess the expression of the reported genes after ( A ) transfection with siRNAs against EPB41L4A-AS1, ( B ) CRISPRa with guides targeting the EPB41L4A-AS1 promoter, ( C ) transfection with plasmid encoding the EPB41L4A-AS1 cDNA, transfection with GapmeRs ( D ) and siRNAs ( E ) targeting EPB41L4A, ( F ) CRISPRa with guides targeting the EPB41L4A promoter, and ( G ) transfection with plasmid encoding the EPB41L4A cDNA. ( H ) Changes in EPB41L4A-AS1 expression after rescuing EPB41L4A-AS1 with an ectopic plasmid or CRISPRa following its KD with GapmeRs. In both panels (Ectopic OE and CRISPRa) the ‘-’ samples represent those transfected with the Empty Vector or sgControl. Asterisks indicate significance relative to the –/– control (transfected with both the control GapmeR and vector). ( I ) Same as in ( H ), but for changes in EPB41L4A expression. ( J ) UMI-4C contact profiles in control and LNA2-transfected cells using baits targeting the TSS of EPB41L4A-AS1. The green area represents the quantified genomic interval, and the p-value was calculated using a Chi-squared test. All experiments were performed in n = 3 biological replicates, except UMI-4C with n = 2, with the error bars in the boxplots representing the standard deviation. In all cases, ns = p > 0.05; *p < 0.05; **p < 0.01; ***p < 0.001 (two-sided Student’s t -test).

    Article Snippet: RNA was then retrotranscribed using the qScript Flex cDNA Synthesis Kit (Quanta Bio), following standard conditions as suggested by the manufacturer.

    Techniques: Quantitative RT-PCR, Expressing, Transfection, Plasmid Preparation, Control, Standard Deviation